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| Sukprasert,S; Uawonggul,N; Jamjanya,T; Thammasirirak,S; Daduang,J; Daduang,S. |
| Sol i 2 is a potent allergen in Solenopsis invicta venom, and most humans exhibit reactivity to it. The Sol gem 2 allergen found in the venom of the Thai tropical fire ant Solenopsis geminata was analysed in the present study. The protein was present in higher amounts than other proteins, as determined by SDS-PAGE, and presumably has allergenic properties similar to those of Sol i 2. Sol gem 2 molecular weight is 28 and 15 kDa, respectively, under non-reducing and reducing conditions, indicating that its native form is a dimer. LC-MS/MS analysis confirmed its similarity to Sol i 2. The mono/dimeric form of Sol gem 2 was determined to be relevant by proteomic approach and immunoblotting. An anti-Sol gem 2 antibody was produced in mice, with a titer greater... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Allergy; Ant venom; Hymenoptera; Immunoreactivity. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000300010 |
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| Orsi,R. O.; Sforcin,J. M.; Funari,S. R. C.; Gomes,J. C.. |
| The direct effect of ethanolic extract of propolis on guinea pig lung cell suspension containing mast cells, as well as its influence on the histamine release induced by antigen (ovoalbumin 10 mug/ml) and ionophore A 23187 (3 muM) were investigated. Propolis ethanolic extract (300 mug/ml) increased the histamine release in guinea pig lung suspension containing mast cells by a cytotoxic effect. Lower concentrations of propolis had no effect on histamine release. Our results demonstrated that propolis (3, 10, 30, and 100 mug/ml) shows no significant effect on the histamine release induced by ionophore and antigen. Based on these results, we suggest that propolis could directly activate mast cells, promoting inflammatory mediators release by cytotoxic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mast cells; Allergy; Histamine; Propolis. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100009 |
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| Albuquerque,A.A.S.; Ferreira,L.G.; Carvalho,M.T.M.; Capellini,V.K.; Evora,P.R.B.; Celotto,A.C.. |
| Anaphylactic shock can be defined as an acute syndrome, and it is the most severe clinical manifestation of allergic diseases. Anaphylactoid reactions are similar to anaphylactic events but differ in the pathophysiological mechanism. Nitric oxide (NO) inhibitors during anaphylaxis suggest that NO might decrease the signs and symptoms of anaphylaxis but exacerbate associated vasodilation. Therefore, blocking the effects of NO on vascular smooth muscle by inhibiting the guanylate cyclase (GC) would be a reasonable strategy. This study aimed to investigate the effects of NO/cGMP pathway inhibitors methylene blue (MB), Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME), and indigo carmine (IC) in shock induced by compound 48/80 (C48/80) in rats. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Endothelial cells; Allergy; Rodent; Nitric oxide; Anaphylaxis. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000300606 |
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| Maciel,M.; Fusaro,A.E.; Oliveira,C.R.; Futata,E.A.; Duarte,A.J.S.; Sato,M.N.. |
| Induced oral tolerance to mucosal-exposed antigens in immunized animals is of particular interest for the development of immunotherapeutic approaches to human allergic diseases. This is a unique feature of mucosal surfaces which represent the main contact interface with the external environment. However, the influence of oral tolerance on specific and natural polyreactive IgA antibodies, the major defense mechanism of the mucosa, is unknown. We have shown that oral administration of an extract of the dust mite Dermatophagoides pteronyssinus (Dp) to primed mice caused down-regulation of IgE responses and an increase in tumor growth factor-ß secretion. In the present study, we observed that primed inbred female A/Sn mice (8 to 10 weeks old) fed by gavage a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mucosa; Atural IgA antibodies; Oral tolerance; Cytokines; Allergy; Dermatophagoides pteronyssinus. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600006 |
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| Brito,F.A.; Lima,L.A.; Ramos,M.F.S.; Nakamura,M.J.; Cavalher-Machado,S.C.; Siani,A.C.; Henriques,M.G.M.O.; Sampaio,A.L.F.. |
| Myrtaceae is a plant family widely used in folk medicine and Syzygium and Eugenia are among the most important genera. We investigated the anti-allergic properties of an aqueous leaf extract of Syzygium cumini (L.) Skeels (SC). HPLC analysis revealed that hydrolyzable tannins and flavonoids are the major components of the extract. Oral administration of SC (25-100 mg/kg) in Swiss mice (20-25 g; N = 7/group) inhibited paw edema induced by compound 48/80 (50% inhibition, 100 mg/kg; P <= 0.05) and, to a lesser extent, the allergic paw edema (23% inhibition, 100 mg/kg; P <= 0.05). SC treatment also inhibited the edema induced by histamine (58% inhibition; P <= 0.05) and 5-HT (52% inhibition; P <= 0.05) but had no effect on platelet-aggregating... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Myrtaceae; Syzygium cumini; Allergy; Paw edema; Pleurisy; Cytokine. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100014 |
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| Oliveira,Aristeu José de; Daemon,Erik. |
| From July to September, 2000 (winter), and from January to March, 2001 (summer), 30 dust samples were collected for each season, from beds of rural dwellings located in farms in the geographical area named "Zona da Mata", Minas Gerais, Brazil. After being sorted, the mites were identified and quantified. The prevalence of mites in the samples was 100%. 891 mites were found in winter (22.97%), and 2988 in summer (77.03%). In winter, Dermatophagoides pteronyssinus (Trouessart, 1897) was the most prevalent (55.00%), followed by Blomia tropicalis (Bronswijk, Cock & Oshima, 1973) (27.06%), Euroglyphus maynei (Cooreman, 1950) (8.85%), and predator mites from Cheyletidae family (8.07%). In summer, the most prevalent species was B. tropicalis (47.79%),... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Air humidity; Allergens; Allergy; Beds; Countryside; Seasonal variations. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752003000400020 |
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| Almeida,K.C.; Silva,D.A.O.; Gennari-Cardoso,M.L.; Cunha-Júnior,J.P.; Alves,R.; Ynoue,L.H.; Resende,R.O.; Sung,S.J.; Taketomi,E.A.. |
| Blomia tropicalis (Bt) and Dermatophagoides pteronyssinus (Dp) are the prevalent house dust mites in tropical countries and are associated with allergic diseases. Glycosylated antigens are highly immunogenic and involved in different pathologies. We evaluated the presence of IgE, IgG1, and IgG4 to concanavalin A-binding antigens (Bt-Con-A) isolated from Bt-total extract in sera of allergic and non-allergic subjects. Bt-total and Bt-Con-A extracts were evaluated by SDS-PAGE and ELISA for reacting with IgE, IgG1, and IgG4 in sera of 121 patients with allergic rhinitis and 36 non-allergic individuals. All subjects were skin prick tested with Bt-total extract and inhibition tests were performed for IgE, IgG1, and IgG4 using both extracts (Bt-total and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Allergy; Blomia tropicalis; Concanavalin A; Immunoglobulin E; Immunoglobulin G1; Immunoglobulin G4. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001100008 |
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